@article{921,
  keywords = {Human immunodeficiency virus, Human papillomavirus, Hybrid capture-2, Xpert},
  author = {Zizipho Mbulawa and Timothy Wilkin and Bridgette Goeieman and Avril Swarts and Sophie Williams and Simon Levin and Mark Faesen and Jennifer Smith and Carla Chibwesha and Anna-Lise Williamson and Cynthia Firnhaber},
  title = {Xpert human papillomavirus test is a promising cervical cancer screening test for HIV-seropositive women.},
  abstract = {<p><strong>UNLABELLED: </strong>This study investigated the performance of Cepheid Xpert human papillomavirus (HPV) assay in South African human immunodeficiency virus (HIV)-infected women and compared its performance with that of hybrid capture-2 (hc2).</p><p><strong>METHODS: </strong>Stored cervical specimens from HIV-infected women that had previously been tested using hc2 were tested using Xpert.</p><p><strong>RESULTS: </strong>The overall HR-HPV prevalence was found to be 62.0\% (720/1161) by Xpert and 61.2\% (711/1161) by hc2. 13.6\% (158/1161) were HPV16 positive, 18.8\% (218/1161) were HPV18/45, 37.3\% (434/1161) were HPV31/33/35/52/58, 12.7\% (147/1161) were HPV51/59 and 23.3\% (270/1161) were HPV39/68/56/66. Overall agreement with hc2 was 90\%; Cohen{\textquoteright}s kappa was 0.78 (95\% CI 0.74-0.82) indicating substantial agreement. Detection of HPV16, HPV18/45, and HPV31/33/35/52/58 were independently associated with cervical intraepithelial neoplasia (CIN)-2+ (P\&lt;0.0001 for each); while HPV51/59 and HPV39/68/56/66 were not. Women infected with HPV16, HPV18/45 or HPV31/33/35/52/58 were found to have significantly higher amounts of HPV DNA detected for those with CIN2+ compared to those without CIN2+, P\&lt;0.0001 for each. Xpert and hc2 were similarly sensitive (88.3\% and 91.5\%, respectively) and specific (48.4\% and 51.0\%) for CIN2+ and CIN3 (sensitivity: 95.8\% and 97.9\%; specificity: 41.4\% and 42.8\%).</p><p><strong>CONCLUSIONS: </strong>Xpert is a promising screening test in HIV-infected women that performs similarly to hc2.</p>},
  year = {2016},
  journal = {Papillomavirus research (Amsterdam, Netherlands)},
  volume = {2},
  pages = {56-60},
  month = {12/2016},
  issn = {2405-8521},
  doi = {10.1016/j.pvr.2016.02.004},
  language = {eng},
}